Primary Protein Structure

Summary

The primary structure of proteins refers to the sequence or chain of amino acids. These amino acids are linked together by peptide bonds.

Key Points

• Primary Structure
  • sequence of amino acids linked together by covalent peptide (amide) bonds
    • Affects protein orientation via rotation of N-C and C-C bonds 
  • Factors affecting primary structure:
    • Protease cleavage of peptide bonds
    • DNA mutagenesis (different amino acids encoded)
  • Note: Protein denaturation (unfolding, loss of 3D shape) does not alter primary structure
    • primary sequence of amino acids is unchanged